Tissue imaging with coherent anti-Stokes Raman scattering microscopy
نویسنده
چکیده
Sir Chandrasekhara Venkata Raman was justifiably excited when he noticed that when a monochromatic light beam is passed through a simple transparent liquid, different colors can be detected in the scattered light. Raman called this frequency-shifted scattered light ‘a new type of secondary radiation’, because he realized that the phenomenon he discovered was caused by a molecular property different from the property of fluorescence emission. Since Raman’s early work in 1928, it has been well established that molecular vibrations are responsible for the observed frequency-shifts in the scattered light. The discovery of the Raman effect was only the beginning of a broad and growing branch of molecular spectroscopy, with new applications that continue to emerge to this day, some of which are presented in this book.
منابع مشابه
Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications
Coherent anti-Stokes Raman scattering (CARS) microscopy permits vibrational imaging with high-sensitivity, high speed, and three-dimensional spatial resolution. We review recent advances in CARS microscopy, including experimental design, theoretical understanding of contrast mechanisms, and applications to chemical and biological systems. We also review the development of multiplex CARS microsp...
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